| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Institute for Adult Disease, Asahi Life Foundation, Tokyo 116; 2 Department of Internal Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113; 3 Department of Internal Medicine, University of Tohoku, Sendai 980-8575; and 4 Fourth Department of Internal Medicine, Saitama Medical School, Moroyama, Saitama, Japan
5-Aminoimidazole-4-carboxamide
ribonucleoside (AICAR) reportedly activates AMP-activated protein
kinase (AMPK) and stimulates glucose uptake by skeletal muscle cells.
In this study, we investigated the role of AMPK in AICAR-induced
glucose uptake by 3T3-L1 adipocytes and rat soleus muscle cells by
overexpressing wild-type and dominant negative forms of the AMPK
2
subunit by use of adenovirus-mediated gene transfer. Overexpression of
the dominant negative mutant had no effect on AICAR-induced glucose
transport in adipocytes, although AMPK activation was almost completely
abolished. This suggests that AICAR-induced glucose uptake by 3T3-L1
adipocytes is independent of AMPK activation. By contrast,
overexpression of the dominant negative AMPK2 mutant in muscle
markedly suppressed both AICAR-induced glucose uptake and AMPK
activation, although insulin-induced uptake was unaffected.
Overexpression of the wild-type AMPK2 subunit significantly
increased AMPK activity in muscle but did not enhance glucose uptake.
Thus, although AMPK activation may not, by itself, be sufficient to
increase glucose transport, it appears essential for AICAR-induced
glucose uptake in muscle.
AMP-activated protein kinase; 5-aminoimidazole-4-carboxamide ribonucleoside; exercise
This article has been cited by other articles:
|
|
 |
|
  R. Saeedi, H. L. Parsons, R. B. Wambolt, K. Paulson, V. Sharma, J. R. B. Dyck, R. W. Brownsey, and M. F. Allard Metabolic actions of metformin in the heart can occur by AMPK-independent mechanisms Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2497 - H2506. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Fujii, N. Jessen, and L. J. Goodyear AMP-activated protein kinase and the regulation of glucose transport Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E867 - E877. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Daval, F. Foufelle, and P. Ferre Functions of AMP-activated protein kinase in adipose tissue J. Physiol., July 1, 2006; 574(1): 55 - 62. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Guigas, L. Bertrand, N. Taleux, M. Foretz, N. Wiernsperger, D. Vertommen, F. Andreelli, B. Viollet, and L. Hue 5-Aminoimidazole-4-Carboxamide-1-{beta}-D-Ribofuranoside and Metformin Inhibit Hepatic Glucose Phosphorylation by an AMP-Activated Protein Kinase-Independent Effect on Glucokinase Translocation. Diabetes, April 1, 2006; 55(4): 865 - 874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Hutchinson and T. Bengtsson AMP-Activated Protein Kinase Activation by Adrenoceptors in L6 Skeletal Muscle Cells: Mediation by {alpha}1-Adrenoceptors Causing Glucose Uptake Diabetes, March 1, 2006; 55(3): 682 - 690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kukidome, T. Nishikawa, K. Sonoda, K. Imoto, K. Fujisawa, M. Yano, H. Motoshima, T. Taguchi, T. Matsumura, and E. Araki Activation of AMP-Activated Protein Kinase Reduces Hyperglycemia-Induced Mitochondrial Reactive Oxygen Species Production and Promotes Mitochondrial Biogenesis in Human Umbilical Vein Endothelial Cells Diabetes, January 1, 2006; 55(1): 120 - 127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Igata, H. Motoshima, K. Tsuruzoe, K. Kojima, T. Matsumura, T. Kondo, T. Taguchi, K. Nakamaru, M. Yano, D. Kukidome, et al. Adenosine Monophosphate-Activated Protein Kinase Suppresses Vascular Smooth Muscle Cell Proliferation Through the Inhibition of Cell Cycle Progression Circ. Res., October 14, 2005; 97(8): 837 - 844. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yamaguchi, H. Katahira, S. Ozawa, Y. Nakamichi, T. Tanaka, T. Shimoyama, K. Takahashi, K. Yoshimoto, M. O. Imaizumi, S. Nagamatsu, et al. Activators of AMP-activated protein kinase enhance GLUT4 translocation and its glucose transport activity in 3T3-L1 adipocytes Am J Physiol Endocrinol Metab, October 1, 2005; 289(4): E643 - E649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sakoda, M. Fujishiro, J. Fujio, N. Shojima, T. Ogihara, A. Kushiyama, Y. Fukushima, M. Anai, H. Ono, M. Kikuchi, et al. Glycogen debranching enzyme association with {beta}-subunit regulates AMP-activated protein kinase activity Am J Physiol Endocrinol Metab, September 1, 2005; 289(3): E474 - E481. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Vankoningsloo, M. Piens, C. Lecocq, A. Gilson, A. De Pauw, P. Renard, C. Demazy, A. Houbion, M. Raes, and T. Arnould Mitochondrial dysfunction induces triglyceride accumulation in 3T3-L1 cells: role of fatty acid {beta}-oxidation and glucose J. Lipid Res., June 1, 2005; 46(6): 1133 - 1149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Smith, C. R. Bruce, and D. J. Dyck AMP kinase activation with AICAR further increases fatty acid oxidation and blunts triacylglycerol hydrolysis in contracting rat soleus muscle J. Physiol., June 1, 2005; 565(2): 547 - 553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ishigaki, H. Katagiri, T. Yamada, T. Ogihara, J. Imai, K. Uno, Y. Hasegawa, J. Gao, H. Ishihara, T. Shimosegawa, et al. Dissipating Excess Energy Stored in the Liver Is a Potential Treatment Strategy for Diabetes Associated With Obesity Diabetes, February 1, 2005; 54(2): 322 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Al-Khalili, A. Krook, J. R. Zierath, and G. D. Cartee Prior serum- and AICAR-induced AMPK activation in primary human myocytes does not lead to subsequent increase in insulin-stimulated glucose uptake Am J Physiol Endocrinol Metab, September 1, 2004; 287(3): E553 - E557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wu, H. Motoshima, K. Mahadev, T. J. Stalker, R. Scalia, and B. J. Goldstein Involvement of AMP-Activated Protein Kinase in Glucose Uptake Stimulated by the Globular Domain of Adiponectin in Primary Rat Adipocytes Diabetes, June 1, 2003; 52(6): 1355 - 1363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Koistinen, D. Galuska, A. V. Chibalin, J. Yang, J. R. Zierath, G. D. Holman, and H. Wallberg-Henriksson 5-Amino-Imidazole Carboxamide Riboside Increases Glucose Transport and Cell-Surface GLUT4 Content in Skeletal Muscle From Subjects With Type 2 Diabetes Diabetes, May 1, 2003; 52(5): 1066 - 1072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. P. Wojtaszewski, C. MacDonald, J. N. Nielsen, Y. Hellsten, D. G. Hardie, B. E. Kemp, B. Kiens, and E. A. Richter Regulation of 5'AMP-activated protein kinase activity and substrate utilization in exercising human skeletal muscle Am J Physiol Endocrinol Metab, April 1, 2003; 284(4): E813 - E822. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
