AJP - Endo Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 282: E197-E206, 2002;
0193-1849/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Recchia, F. A.
Right arrow Articles by Stanley, W. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Recchia, F. A.
Right arrow Articles by Stanley, W. C.
Vol. 282, Issue 1, E197-E206, January 2002

Reduced synthesis of NO causes marked alterations in myocardial substrate metabolism in conscious dogs

Fabio A. Recchia1, Juan Carlos Osorio1, Margaret P. Chandler2, Xiaobin Xu1, Ashish R. Panchal2, Gary D. Lopaschuk3, Thomas H. Hintze1, and William C. Stanley2

1 Department of Physiology, New York Medical College, Valhalla, New York 10595; 2 Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio 44106; and 3 Heritage Medical Research Centre, University of Alberta, Edmonton, Canada T6G 2S2

To test whether the acute reduction of nitric oxide (NO) synthesis causes changes in cardiac substrate metabolism and in the activity of key enzymes of fatty acid and glucose oxidation, we blocked NOS by giving Nomega -nitro-L-arginine methyl ester (L-NAME; 35 mg/kg iv two times) to nine chronically instrumented dogs. [3H]oleate, [14C]glucose, and [13C]lactate were infused to measure the rate of cardiac substrate uptake and oxidation. Glyceraldehyde-3-phosphate dehydrogenase, acetyl-CoA carboxylase, and malonyl-CoA decarboxylase activities were measured in myocardial biopsies. In eight control dogs, ANG II was infused (20-40 ng · kg-1 · min-1) to mimic the hemodynamic effects of L-NAME. After L-NAME, significant changes occurred for fatty acid oxidation (from 9.8 ± 0.8 to 7.1 ± 1.2 µmol/min), glucose uptake (from 12.9 ± 5.5 to 45.0 ± 14.2 µmol/min), and oxidation (from 4.4 ± 1.2 to 19.9 ± 2.3 µmol/min). ANG caused only a significantly lower increase in glucose oxidation. Lactate uptake increased by more than twofold in both groups. The enzyme activities did not differ significantly between the two groups. In conclusion, the acute inhibition of NO synthesis causes marked metabolic alterations that do not involve key rate-controlling enzymes of fatty acid oxidation nor glyceraldehyde-3-phosphate dehydrogenase.

fatty acids; glucose; lactate; oxidation; heart


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
P. F. Soto, P. Herrero, K. B. Schechtman, A. D. Waggoner, J. M. Baumstark, A. A. Ehsani, and R. J. Gropler
Exercise training impacts the myocardial metabolism of older individuals in a gender-specific manner
Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H842 - H850.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. G. Williams, C. Ojaimi, K. Qanud, S. Zhang, X. Xu, F. A. Recchia, and T. H. Hintze
Coronary nitric oxide production controls cardiac substrate metabolism during pregnancy in the dog
Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2516 - H2523.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
E. Murphy and C. Steenbergen
Mechanisms Underlying Acute Protection From Cardiac Ischemia-Reperfusion Injury
Physiol Rev, April 1, 2008; 88(2): 581 - 609.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. Neglia, A. De Caterina, P. Marraccini, A. Natali, M. Ciardetti, C. Vecoli, A. Gastaldelli, D. Ciociaro, P. Pellegrini, R. Testa, et al.
Impaired myocardial metabolic reserve and substrate selection flexibility during stress in patients with idiopathic dilated cardiomyopathy
Am J Physiol Heart Circ Physiol, December 1, 2007; 293(6): H3270 - H3278.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
A. L. Taylor, S. Ziesche, C. W. Yancy, P. Carson, K. Ferdinand, M. Taylor, K. Adams, A. Y. Olukotun, E. Ofili, S. W. Tam, et al.
Early and Sustained Benefit on Event-Free Survival and Heart Failure Hospitalization From Fixed-Dose Combination of Isosorbide Dinitrate/Hydralazine: Consistency Across Subgroups in the African-American Heart Failure Trial
Circulation, April 3, 2007; 115(13): 1747 - 1753.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. d'Agostino, V. Labinskyy, V. Lionetti, M. P. Chandler, B. Lei, K. Matsuo, M. Bellomo, X. Xu, T. H. Hintze, W. C. Stanley, et al.
Altered cardiac metabolic phenotype after prolonged inhibition of NO synthesis in chronically instrumented dogs
Am J Physiol Heart Circ Physiol, April 1, 2006; 290(4): H1721 - H1726.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
K. L. King, I. C. Okere, N. Sharma, J. R. B. Dyck, A. E. Reszko, T. A. McElfresh, J. Kerner, M. P. Chandler, G. D. Lopaschuk, and W. C. Stanley
Regulation of cardiac malonyl-CoA content and fatty acid oxidation during increased cardiac power
Am J Physiol Heart Circ Physiol, September 1, 2005; 289(3): H1033 - H1037.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
W. C. Stanley, F. A. Recchia, and G. D. Lopaschuk
Myocardial Substrate Metabolism in the Normal and Failing Heart
Physiol Rev, July 1, 2005; 85(3): 1093 - 1129.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
N. Sharma, I. C. Okere, D. Z. Brunengraber, T. A. McElfresh, K. L. King, J. P. Sterk, H. Huang, M. P. Chandler, and W. C. Stanley
Regulation of pyruvate dehydrogenase activity and citric acid cycle intermediates during high cardiac power generation
J. Physiol., January 15, 2005; 562(2): 593 - 603.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Zhang, G. Gong, Y. Ye, T. Guo, A. Mansoor, Q. Hu, K. Ochiai, J. Liu, X. Wang, Y. Cheng, et al.
Nitric oxide regulation of myocardial O2 consumption and HEP metabolism
Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H310 - H316.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
A. L. Taylor, S. Ziesche, C. Yancy, P. Carson, R. D'Agostino Jr., K. Ferdinand, M. Taylor, K. Adams, M. Sabolinski, M. Worcel, et al.
Combination of Isosorbide Dinitrate and Hydralazine in Blacks with Heart Failure
N. Engl. J. Med., November 11, 2004; 351(20): 2049 - 2057.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. Li, X. Hu, P. Selvakumar, R. R. Russell III, S. W. Cushman, G. D. Holman, and L. H. Young
Role of the nitric oxide pathway in AMPK-mediated glucose uptake and GLUT4 translocation in heart muscle
Am J Physiol Endocrinol Metab, November 1, 2004; 287(5): E834 - E841.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
W. C. Stanley, S. R. Meadows, K. M. Kivilo, B. A. Roth, and G. D. Lopaschuk
{beta}-Hydroxybutyrate inhibits myocardial fatty acid oxidation in vivo independent of changes in malonyl-CoA content
Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1626 - H1631.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. Linke, G. Zhao, F. A. Recchia, J. Williams, X. Xu, and T. H. Hintze
Shift in metabolic substrate uptake by the heart during development of alloxan-induced diabetes
Am J Physiol Heart Circ Physiol, August 7, 2003; 285(3): H1007 - H1014.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Z. Z. Kojic, U. Flogel, J. Schrader, and U. K. M. Decking
Endothelial NO formation does not control myocardial O2 consumption in mouse heart
Am J Physiol Heart Circ Physiol, June 5, 2003; 285(1): H392 - H397.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. Martin, R. Schulz, H. Post, P. Gres, and G. Heusch
Effect of NO synthase inhibition on myocardial metabolism during moderate ischemia
Am J Physiol Heart Circ Physiol, June 1, 2003; 284(6): H2320 - H2324.
[Abstract] [Full Text] [PDF]

Home page
 Eur Heart J SupplHome page
Z. He, C. Rask-Madsen, and G.L. King
Mechanisms of cardiovascular complications in diabetes and potential new pharmacological therapies
Eur. Heart J. Suppl., January 1, 2003; 5(suppl_B): B51 - B57.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online