AJP - Endo Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 282: E18-E23, 2002;
0193-1849/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (91)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fisher, J. S.
Right arrow Articles by Nolte, L. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fisher, J. S.
Right arrow Articles by Nolte, L. A.
Vol. 282, Issue 1, E18-E23, January 2002

Activation of AMP kinase enhances sensitivity of muscle glucose transport to insulin

Jonathan S. Fisher, Jiaping Gao, Dong-Ho Han, John O. Holloszy, and Lorraine A. Nolte

Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

Evidence has accumulated that activation of AMP kinase (AMPK) mediates the acute increase in glucose transport induced by exercise. As the exercise-induced, insulin-independent increase in glucose transport wears off, it is followed by an increase in muscle insulin sensitivity. The major purpose of this study was to determine whether hypoxia and 5-aminoimidazole-4-carboxamide-1-beta -D-ribofuranoside (AICAR), which also activate AMPK and stimulate glucose transport, also induce an increase in insulin sensitivity. We found that the increase in glucose transport in response to 30 µU/ml insulin was about twofold greater in rat epitrochlearis muscles that had been made hypoxic or treated with AICAR 3.5 h previously than in untreated control muscles. This increase in insulin sensitivity was similar to that induced by a 2-h bout of swimming or 10 min of in vitro electrically stimulated contractions. Neither phosphatidylinositol 3-kinase activity nor protein kinase B (PKB) phosphorylation in response to 30 µU/ml insulin was enhanced by prior exercise or AICAR treatment that increased insulin sensitivity of glucose transport. Inhibition of protein synthesis by inclusion of cycloheximide in the incubation medium for 3.5 h after exercise did not prevent the increase in insulin sensitivity. Contractions, hypoxia, and treatment with AICAR all caused a two- to three-fold increase in AMPK activity over the resting level. These results provide evidence that the increase in insulin sensitivity of muscle glucose transport that follows exercise is mediated by activation of AMPK and involves a step beyond PKB in the pathway by which insulin stimulates glucose transport.

cycloheximide; exercise; hypoxia; insulin signaling; 5-aminoimidazole-4-carboxamide-1-beta -D-ribofuranoside


This article has been cited by other articles:


Home page
 DiabetesHome page
N. Fujii, R. C. Ho, Y. Manabe, N. Jessen, T. Toyoda, W. L. Holland, S. A. Summers, M. F. Hirshman, and L. J. Goodyear
Ablation of AMP-Activated Protein Kinase {alpha}2 Activity Exacerbates Insulin Resistance Induced by High-Fat Feeding of Mice
Diabetes, November 1, 2008; 57(11): 2958 - 2966.
[Abstract] [Full Text] [PDF]

Home page
 ptjournalHome page
L. P Turcotte and J. S Fisher
Skeletal Muscle Insulin Resistance: Roles of Fatty Acid Metabolism and Exercise
Physical Therapy, November 1, 2008; 88(11): 1279 - 1296.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
B. K. Pedersen and M. A. Febbraio
Muscle as an Endocrine Organ: Focus on Muscle-Derived Interleukin-6
Physiol Rev, October 1, 2008; 88(4): 1379 - 1406.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. J. Zhu, B. Han, J. Tong, C. Ma, J. M. Kimzey, K. R. Underwood, Y. Xiao, B. W. Hess, S. P. Ford, P. W. Nathanielsz, et al.
AMP-activated protein kinase signalling pathways are down regulated and skeletal muscle development impaired in fetuses of obese, over-nourished sheep
J. Physiol., May 15, 2008; 586(10): 2651 - 2664.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
X. Ruan, F. Zheng, and Y. Guan
PPARs and the kidney in metabolic syndrome
Am J Physiol Renal Physiol, May 1, 2008; 294(5): F1032 - F1047.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Dominguez, P. Wu, C. S. Packer, C. Temm, and K. J. Kelly
Lipotoxic and inflammatory phenotypes in rats with uncontrolled metabolic syndrome and nephropathy
Am J Physiol Renal Physiol, September 1, 2007; 293(3): F670 - F679.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
E. J. Kim, S.-N. Jung, K. H. Son, S. R. Kim, T. Y. Ha, M. G. Park, I. G. Jo, J. G. Park, W. Choe, S.-S. Kim, et al.
Antidiabetes and Antiobesity Effect of Cryptotanshinone via Activation of AMP-Activated Protein Kinase
Mol. Pharmacol., July 1, 2007; 72(1): 62 - 72.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
P. C. Geiger, C. Hancock, D. C. Wright, D.-H. Han, and J. O. Holloszy
IL-6 increases muscle insulin sensitivity only at superphysiological levels
Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1842 - E1846.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
T. Shibakusa, W. Mizunoya, Y. Okabe, S. Matsumura, Y. Iwaki, A. Okuno, K. Shibata, K. Inoue, and T. Fushiki
Transforming growth factor-beta in the brain is activated by exercise and increases mobilization of fat-related energy substrates in rats
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2007; 292(5): R1851 - R1861.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
E. B. Arias, J. Kim, K. Funai, and G. D. Cartee
Prior exercise increases phosphorylation of Akt substrate of 160 kDa (AS160) in rat skeletal muscle
Am J Physiol Endocrinol Metab, April 1, 2007; 292(4): E1191 - E1200.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. P. Thyfault, M. G. Cree, D. Zheng, J. J. Zwetsloot, E. B. Tapscott, T. R. Koves, O. Ilkayeva, R. R. Wolfe, D. M. Muoio, and G. L. Dohm
Contraction of insulin-resistant muscle normalizes insulin action in association with increased mitochondrial activity and fatty acid catabolism
Am J Physiol Cell Physiol, February 1, 2007; 292(2): C729 - C739.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J.-S. Ju, M. A. Gitcho, C. A. Casmaer, P. B. Patil, D.-G. Han, S. A. Spencer, and J. S. Fisher
Potentiation of insulin-stimulated glucose transport by the AMP-activated protein kinase
Am J Physiol Cell Physiol, January 1, 2007; 292(1): C564 - C572.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
J. B. Birk and J. F. P. Wojtaszewski
Predominant {alpha}2/{beta}2/{gamma}3 AMPK activation during exercise in human skeletal muscle
J. Physiol., December 15, 2006; 577(3): 1021 - 1032.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
P. C. Geiger, D. H. Han, D. C. Wright, and J. O. Holloszy
How muscle insulin sensitivity is regulated: testing of a hypothesis
Am J Physiol Endocrinol Metab, December 1, 2006; 291(6): E1258 - E1263.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. Hamada, E. B. Arias, and G. D. Cartee
Increased submaximal insulin-stimulated glucose uptake in mouse skeletal muscle after treadmill exercise
J Appl Physiol, November 1, 2006; 101(5): 1368 - 1376.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
N. Fujii, N. Jessen, and L. J. Goodyear
AMP-activated protein kinase and the regulation of glucose transport
Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E867 - E877.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
A. L. Carey, G. R. Steinberg, S. L. Macaulay, W. G. Thomas, A. G. Holmes, G. Ramm, O. Prelovsek, C. Hohnen-Behrens, M. J. Watt, D. E. James, et al.
Interleukin-6 Increases Insulin-Stimulated Glucose Disposal in Humans and Glucose Uptake and Fatty Acid Oxidation In Vitro via AMP-Activated Protein Kinase.
Diabetes, October 1, 2006; 55(10): 2688 - 2697.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
N. K. LeBrasseur, M. Kelly, T.-S. Tsao, S. R. Farmer, A. K. Saha, N. B. Ruderman, and E. Tomas
Thiazolidinediones can rapidly activate AMP-activated protein kinase in mammalian tissues
Am J Physiol Endocrinol Metab, July 1, 2006; 291(1): E175 - E181.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. Sriwijitkamol, J. L. Ivy, C. Christ-Roberts, R. A. DeFronzo, L. J. Mandarino, and N. Musi
LKB1-AMPK signaling in muscle from obese insulin-resistant Zucker rats and effects of training
Am J Physiol Endocrinol Metab, May 1, 2006; 290(5): E925 - E932.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
M. W. Sun, J. Y. Lee, P. I.W. de Bakker, N. P. Burtt, P. Almgren, L. Rastam, T. Tuomi, D. Gaudet, M. J. Daly, J. N. Hirschhorn, et al.
Haplotype Structures and Large-Scale Association Testing of the 5' AMP-Activated Protein Kinase Genes PRKAA2, PRKAB1, and PRKAB2 With Type 2 Diabetes
Diabetes, March 1, 2006; 55(3): 849 - 855.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
D. G. Hardie and K. Sakamoto
AMPK: A Key Sensor of Fuel and Energy Status in Skeletal Muscle
Physiology, February 1, 2006; 21(1): 48 - 60.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
F. Blaschke, Y. Takata, E. Caglayan, R. E. Law, and W. A. Hsueh
Obesity, Peroxisome Proliferator-Activated Receptor, and Atherosclerosis in Type 2 Diabetes
Arterioscler. Thromb. Vasc. Biol., January 1, 2006; 26(1): 28 - 40.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. S. Fisher, J.-S. Ju, P. J. Oppelt, J. L. Smith, A. Suzuki, and H. Esumi
Muscle contractions, AICAR, and insulin cause phosphorylation of an AMPK-related kinase
Am J Physiol Endocrinol Metab, December 1, 2005; 289(6): E986 - E992.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
M. C. Onan, J. S. Fisher, J.-S. Ju, B. C. Fuchs, and B. P. Bode
Type I Diabetes Affects Skeletal Muscle Glutamine Uptake in a Fiber-Specific Manner
Experimental Biology and Medicine, October 1, 2005; 230(9): 606 - 611.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. L. Smith, P. B. Patil, and J. S. Fisher
AICAR and hyperosmotic stress increase insulin-stimulated glucose transport
J Appl Physiol, September 1, 2005; 99(3): 877 - 883.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
C. T. Xiao and J. P. Cant
Relationship Between Glucose Transport and Metabolism in Isolated Bovine Mammary Epithelial Cells
J Dairy Sci, August 1, 2005; 88(8): 2794 - 2805.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. O. Holloszy
Exercise-induced increase in muscle insulin sensitivity
J Appl Physiol, July 1, 2005; 99(1): 338 - 343.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
E. B. Taylor, W. J. Ellingson, J. D. Lamb, D. G. Chesser, and W. W. Winder
Long-chain acyl-CoA esters inhibit phosphorylation of AMP-activated protein kinase at threonine-172 by LKB1/STRAD/MO25
Am J Physiol Endocrinol Metab, June 1, 2005; 288(6): E1055 - E1061.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
J. F. P Wojtaszewski, J. B Birk, C. Frosig, M. Holten, H. Pilegaard, and F. Dela
5'AMP activated protein kinase expression in human skeletal muscle: effects of strength training and type 2 diabetes
J. Physiol., April 15, 2005; 564(2): 563 - 573.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
B. O. Eijnde, W. Derave, J. F. P. Wojtaszewski, E. A. Richter, and P. Hespel
AMP kinase expression and activity in human skeletal muscle: effects of immobilization, retraining, and creatine supplementation
J Appl Physiol, April 1, 2005; 98(4): 1228 - 1233.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
D. K. Kramer, L. Al-Khalili, S. Perrini, J. Skogsberg, P. Wretenberg, K. Kannisto, H. Wallberg-Henriksson, E. Ehrenborg, J. R. Zierath, and A. Krook
Direct Activation of Glucose Transport in Primary Human Myotubes After Activation of Peroxisome Proliferator-Activated Receptor {delta}
Diabetes, April 1, 2005; 54(4): 1157 - 1163.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
P. C. Geiger, D. C. Wright, D.-H. Han, and J. O. Holloszy
Activation of p38 MAP kinase enhances sensitivity of muscle glucose transport to insulin
Am J Physiol Endocrinol Metab, April 1, 2005; 288(4): E782 - E788.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. Shearer, P. T. Fueger, J. N. Rottman, D. P. Bracy, P. H. Martin, and D. H. Wasserman
AMPK stimulation increases LCFA but not glucose clearance in cardiac muscle in vivo
Am J Physiol Endocrinol Metab, November 1, 2004; 287(5): E871 - E877.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
L. Al-Khalili, A. Krook, J. R. Zierath, and G. D. Cartee
Prior serum- and AICAR-induced AMPK activation in primary human myocytes does not lead to subsequent increase in insulin-stimulated glucose uptake
Am J Physiol Endocrinol Metab, September 1, 2004; 287(3): E553 - E557.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
P. Schrauwen and M. K.C. Hesselink
Oxidative Capacity, Lipotoxicity, and Mitochondrial Damage in Type 2 Diabetes
Diabetes, June 1, 2004; 53(6): 1412 - 1417.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. Hamada, T. Hayashi, T. Kimura, K. Nakao, and T. Moritani
Electrical stimulation of human lower extremities enhances energy consumption, carbohydrate oxidation, and whole body glucose uptake
J Appl Physiol, March 1, 2004; 96(3): 911 - 916.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
D.-H. Han, L. A. Nolte, J.-S. Ju, T. Coleman, J. O. Holloszy, and C. F. Semenkovich
UCP-mediated energy depletion in skeletal muscle increases glucose transport despite lipid accumulation and mitochondrial dysfunction
Am J Physiol Endocrinol Metab, March 1, 2004; 286(3): E347 - E353.
[Abstract] [Full Text]

Home page
 J. Appl. Physiol.Home page
J. Kim, R. S. Solis, E. B. Arias, and G. D. Cartee
Postcontraction insulin sensitivity: relationship with contraction protocol, glycogen concentration, and 5' AMP-activated protein kinase phosphorylation
J Appl Physiol, February 1, 2004; 96(2): 575 - 583.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. Hojlund, K. J. Mustard, P. Staehr, D. G. Hardie, H. Beck-Nielsen, E. A. Richter, and J. F. P. Wojtaszewski
AMPK activity and isoform protein expression are similar in muscle of obese subjects with and without type 2 diabetes
Am J Physiol Endocrinol Metab, February 1, 2004; 286(2): E239 - E244.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. B. Jorgensen, B. Viollet, F. Andreelli, C. Frosig, J. B. Birk, P. Schjerling, S. Vaulont, E. A. Richter, and J. F. P. Wojtaszewski
Knockout of the {alpha}2 but Not {alpha}1 5'-AMP-activated Protein Kinase Isoform Abolishes 5-Aminoimidazole-4-carboxamide-1-{beta}-4-ribofuranosidebut Not Contraction-induced Glucose Uptake in Skeletal Muscle
J. Biol. Chem., January 9, 2004; 279(2): 1070 - 1079.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. Suwa, H. Nakano, and S. Kumagai
Effects of chronic AICAR treatment on fiber composition, enzyme activity, UCP3, and PGC-1 in rat muscles
J Appl Physiol, September 1, 2003; 95(3): 960 - 968.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. Hamada, H. Sasaki, T. Hayashi, T. Moritani, and K. Nakao
Enhancement of whole body glucose uptake during and after human skeletal muscle low-frequency electrical stimulation
J Appl Physiol, June 1, 2003; 94(6): 2107 - 2112.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
H. A. Koistinen, D. Galuska, A. V. Chibalin, J. Yang, J. R. Zierath, G. D. Holman, and H. Wallberg-Henriksson
5-Amino-Imidazole Carboxamide Riboside Increases Glucose Transport and Cell-Surface GLUT4 Content in Skeletal Muscle From Subjects With Type 2 Diabetes
Diabetes, May 1, 2003; 52(5): 1066 - 1072.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. O. Holloszy
A forty-year memoir of research on the regulation of glucose transport into muscle
Am J Physiol Endocrinol Metab, March 1, 2003; 284(3): E453 - E467.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. N. Nielsen, K. J. W. Mustard, D. A. Graham, H. Yu, C. S. MacDonald, H. Pilegaard, L. J. Goodyear, D. G. Hardie, E. A. Richter, and J. F. P. Wojtaszewski
5'-AMP-activated protein kinase activity and subunit expression in exercise-trained human skeletal muscle
J Appl Physiol, February 1, 2003; 94(2): 631 - 641.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. H. Park, S. R. Paulsen, S. R. Gammon, K. J. Mustard, D. G. Hardie, and W. W. Winder
Effects of thyroid state on AMP-activated protein kinase and acetyl-CoA carboxylase expression in muscle
J Appl Physiol, December 1, 2002; 93(6): 2081 - 2088.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
G. S. Olsen and B. F. Hansen
AMP kinase activation ameliorates insulin resistance induced by free fatty acids in rat skeletal muscle
Am J Physiol Endocrinol Metab, November 1, 2002; 283(5): E965 - E970.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
M. A. Iglesias, J.-M. Ye, G. Frangioudakis, A. K. Saha, E. Tomas, N. B. Ruderman, G. J. Cooney, and E. W. Kraegen
AICAR Administration Causes an Apparent Enhancement of Muscle and Liver Insulin Action in Insulin-Resistant High-Fat-Fed Rats
Diabetes, October 1, 2002; 51(10): 2886 - 2894.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
E. Tomas, A. Zorzano, and N. B. Ruderman
Exercise Effects on Muscle Insulin Signaling and Action: Exercise and insulin signaling: a historical perspective
J Appl Physiol, August 1, 2002; 93(2): 765 - 772.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. R. Zierath
Exercise Effects of Muscle Insulin Signaling and Action: Invited Review: Exercise training-induced changes in insulin signaling in skeletal muscle
J Appl Physiol, August 1, 2002; 93(2): 773 - 781.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. Sakamoto and L. J. Goodyear
Exercise Effects on Muscle Insulin Signaling and Action: Invited Review: Intracellular signaling in contracting skeletal muscle
J Appl Physiol, July 1, 2002; 93(1): 369 - 383.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. F. P. Wojtaszewski, J. N. Nielsen, and E. A. Richter
Exercise Effects on Muscle Insulin Signaling and Action: Invited Review: Effect of acute exercise on insulin signaling and action in humans
J Appl Physiol, July 1, 2002; 93(1): 384 - 392.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
N. Musi, M. F. Hirshman, J. Nygren, M. Svanfeldt, P. Bavenholm, O. Rooyackers, G. Zhou, J. M. Williamson, O. Ljunqvist, S. Efendic, et al.
Metformin Increases AMP-Activated Protein Kinase Activity in Skeletal Muscle of Subjects With Type 2 Diabetes
Diabetes, July 1, 2002; 51(7): 2074 - 2081.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online