| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Perinatal Research Center and Departments of Pediatrics and 2 Preventive Medicine and Biometrics, University of Colorado Health Sciences Center, Denver, Colorado, 80262; and 3 Department of Surgery, University of Oklahoma College of Medicine, Oklahoma City, Oklahoma, 73190
We studied the effect of an acute 4-h period of
hyperinsulinemia (H) on net utilization rates (AAURnet)
of 21 amino acids (AA) in 17 studies performed in 13 late-gestation
fetal sheep by use of a novel fetal
hyperinsulinemic-euglycemic-euaminoacidemic clamp. During H [84 ± 12 (SE) µU/ml H, 15 ± 2 µU/ml control (C), P < 0.00001], euglycemia was maintained by glucose
clamp (19 ± 0.05 µmol/ml H, 1.19 ± 0.04 µmol/ml C), and
euaminoacidemia (mean 4.1 ± 3.3% increase for all amino acid
concentrations [AA], nonsignificantly different from zero) was
maintained with a mixed amino acid solution adjusted to keep lysine
concentration constant and other [AA] near C values. H produced a
63.7% increase in AAURnet (3.29 ± 0.66 µmol · min
1 · kg1 H,
2.01 ± 0.55 µmol · min1 · kg1 C,
P < 0.001), accounting for a 60.1% increase in fetal
nitrogen uptake rate (2,064 ± 108 mg · day1 · kg1 H,
1,289 ± 73 mg · day1 · kg1 C,
P < 0.001). Mean AA clearance rate
(AAURnet/[AA]) increased by 64.5 ± 18.9%
(P < 0.001). Thus acute physiological H increases net
amino acid and nitrogen utilization rates in the ovine fetus independent of plasma glucose and [AA].
glucose; insulin; amino acids; sheep; nitrogen
This article has been cited by other articles:
|
|
 |
|
  L. D. Brown, P. J. Rozance, J. S. Barry, J. E. Friedman, and W. W. Hay Jr. Insulin is required for amino acid stimulation of dual pathways for translational control in skeletal muscle in the late-gestation ovine fetus Am J Physiol Endocrinol Metab, January 1, 2009; 296(1): E56 - E63. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. N. MacLachlan, S. Hemstreet, B. Matushewski, J. D. McCallum, and B. S. Richardson Induced Hyper and Hypo Amino Acidemia in the Ovine Fetus Near Term: Effects on Electrocortical Activity Reproductive Sciences, September 1, 2008; 15(7): 710 - 719. [Abstract] [PDF]
|
|
|
|
 |
|
 C. J. Valentine and T. D. Puthoff Enhancing Parenteral Nutrition Therapy for the Neonate Nutr Clin Pract, April 1, 2007; 22(2): 183 - 193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. D. Brown and W. W. Hay Jr. Effect of hyperinsulinemia on amino acid utilization and oxidation independent of glucose metabolism in the ovine fetus Am J Physiol Endocrinol Metab, December 1, 2006; 291(6): E1333 - E1340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jozwik, C. Teng, R. B. Wilkening, G. Meschia, and F. C. Battaglia Reciprocal inhibition of umbilical uptake within groups of amino acids Am J Physiol Endocrinol Metab, March 1, 2004; 286(3): E376 - E383. [Abstract] [Full Text]
|
|
|
|
|
|
W. Shen, P. Wisniowski, L. Ahmed, D. W. Boyle, S. C. Denne, and E. A. Liechty Protein anabolic effects of insulin and IGF-I in the ovine fetus Am J Physiol Endocrinol Metab, April 1, 2003; 284(4): E748 - E756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. J. O'Connor, J. A. Bush, A. Suryawan, H. V. Nguyen, and T. A. Davis Insulin and amino acids independently stimulate skeletal muscle protein synthesis in neonatal pigs Am J Physiol Endocrinol Metab, January 1, 2003; 284(1): E110 - E119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Shen, D. Mallon, D. W. Boyle, and E. A. Liechty IGF-I and insulin regulate eIF4F formation by different mechanisms in muscle and liver in the ovine fetus Am J Physiol Endocrinol Metab, September 1, 2002; 283(3): E593 - E603. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Ehrhardt, A. W. Bell, and Y. R. Boisclair Spatial and developmental regulation of leptin in fetal sheep Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2002; 282(6): R1628 - R1635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Stephens, P. J. Thureen, M. L. Goalstone, M. S. Anderson, J. W. Leitner, W. W. Hay Jr., and B. Draznin Fetal hyperinsulinemia increases farnesylation of p21 Ras in fetal tissues Am J Physiol Endocrinol Metab, August 1, 2001; 281(2): E217 - E223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. R. H. Regnault, S. W. Limesand, and W. W. Hay Jr Factors Influencing Fetal Growth NeoReviews, June 1, 2001; 2(6): e119 - 128. [Full Text] [PDF]
|
|
|
|
|
|
T. A. Davis, M. L. Fiorotto, D. G. Burrin, P. J. Reeds, H. V. Nguyen, P. R. Beckett, R. C. Vann, and P. M. J. O'Connor Stimulation of protein synthesis by both insulin and amino acids is unique to skeletal muscle in neonatal pigs Am J Physiol Endocrinol Metab, April 1, 2002; 282(4): E880 - E890. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
