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1 Department of Medicine, McMaster University Medical Centre, Hamilton, Ontario L8N 3Z5; 3 Department of Kinesiology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada; and 2 Department of Clinical Chemistry, Huddinge University Hospital, Karolinska Institute, S-141 86 Huddinge, Sweden
Muscle metabolism,
including the role of pyruvate dehydrogenase (PDH) in muscle lactate
(Lac
) production, was
examined during incremental exercise before and after 7 days of
submaximal training on a cycle ergometer [2 h daily at 60% peak
O2 uptake
(
O2 max)].
Subjects were studied at rest and during continuous steady-state
cycling at three stages (15 min each): 30, 65, and 75% of the
pretraining O2 max.
Blood was sampled from brachial artery and femoral vein, and leg blood flow was measured by thermodilution. Biopsies of the vastus lateralis were obtained at rest and during steady-state exercise at the end of
each stage. O2 max,
leg O2 uptake, and the maximum
activities of citrate synthase and PDH were not altered by training;
muscle glycogen concentration was higher. During rest and cycling at 30% O2 max, muscle
Lac concentration
([Lac]) and leg
efflux were similar. At 65%
O2 max, muscle
[Lac] was lower
(11.9 ± 3.2 vs. 20.0 ± 5.8 mmol/kg dry wt) and
Lac efflux was less
[
0.22 ± 0.24 (one leg) vs. 1.42 ± 0.33 mmol/min] after training. Similarly, at 75%
O2 max, lower muscle
[Lac] (17.2 ± 4.4 vs. 45.2 ± 6.6 mmol/kg dry wt) accompanied less release (0.41 ± 0.53 vs. 1.32 ± 0.65 mmol/min) after training. PDH in its active form
(PDHa) was not different between
conditions. Calculated pyruvate production at 75%
O2 max fell by 33%,
pyruvate reduction to lactate fell by 59%, and pyruvate oxidation fell by 24% compared with before training. Muscle contents of coenzyme A
and phosphocreatine were higher during exercise after training. Lower
muscle lactate production after training resulted from improved matching of glycolytic and PDHa
fluxes, independently of changes in muscle
O2 consumption, and was associated
with greater phosphorylation potential.
lactate; oxygen uptake; pyruvate dehydrogenase; glucose transporters; glycogen; leg blood flow; free fatty acids; phosphorylation potential
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