AJP - Endocrinology and Metabolism, Vol 263, Issue 6 E1035-E1039, Copyright © 1992 by American Physiological Society
Lactate in rat skeletal muscle after hemorrhage measured by microdialysis probe calibrated in situ
C. Okuda, T. Sawa, M. Harada, T. Murakami, T. Matsuda and Y. Tanaka
Department of Anesthesiology, Kyoto Prefectural University of Medicine, Japan.
The interstitial lactate concentration in rat skeletal muscle was measured
in the normal state and after hemorrhage using a microdialysis probe, and
the values calculated by in vitro and in vivo calibration were compared.
After withdrawal of 30% of the estimated total blood volume, the lactate
concentration in the dialysate gradually increased and then maintained an
almost constant level. It was found that interstitial lactate levels of
skeletal muscle calculated by the in vitro calibration method were not
significantly different from those calculated by in vivo calibration in
both the normal state and after hemorrhage. These data indicate that the
absolute lactate concentration in the muscle interstitium in the normal
state and after acute hemorrhage can be practically measured by a
microdialysis probe calibrated in vitro. From the comparison of lactate
concentrations in blood and in the muscle interstitium, it was suggested
that rat skeletal muscle functions as a lactate consumer, rather than as a
lactate producer, after acute hemorrhage.
