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Am J Physiol Endocrinol Metab 261: E354-E361, 1991;
0193-1849/91 $5.00
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AJP - Endocrinology and Metabolism, Vol 261, Issue 3 E354-E361, Copyright © 1991 by American Physiological Society

ARTICLES

Sex-related differences in meprin-A, a membrane-bound mouse kidney proteinase

S. T. Stroupe, S. S. Craig, C. M. Gorbea and J. S. Bond
Department of Biochemistry, Virginia Commonwealth University, Richmond 23298.

To investigate the expression of meprin-A, a brush-border metalloproteinase in mouse tissues, immunohistochemical studies were conducted using a monoclonal antibody prepared against a purified form of kidney meprin-A form male mice. Kidney slices from female mice displayed markedly less immunoreactivity compared with similar preparations from male mice using this antibody. However, the specific activities of meprin-A in kidney homogenates and purified preparations of meprin-A from male and female mice were not significantly different. Western blots of kidney membrane proteins from several mouse strains indicated that the female form of meprin-A had a decreased mobility relative to the male form when subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis; this difference could be eliminated by treatment of preparations with endoglycosidase F, which removes some asparagine-linked oligosaccharides. These data and lectin blots of membrane proteins indicate that there are differences in the glycosylation (specifically in the complex type oligosaccharides) of meprin-A in adult (8 wk old) male and female mice. Juvenile (3 wk old) male and female mice displayed similar amounts of immunohistochemical staining in kidney slices, as well as similar meprin-A electrophoretic mobilities and lectin affinities. Administration of 17 beta-estradiol to gonadectomized adult mice decreased the immunoreactivity of meprin-A in kidney slices and the electrophoretic mobility of meprin-A. These studies indicate that estrogens affect posttranslational modifications of meprin-A.


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L. P. Norman, W. Jiang, X. Han, T. L. Saunders, and J. S. Bond
Targeted Disruption of the Meprin {beta} Gene in Mice Leads to Underrepresentation of Knockout Mice and Changes in Renal Gene Expression Profiles
Mol. Cell. Biol., February 15, 2003; 23(4): 1221 - 1230.
[Abstract] [Full Text] [PDF]


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