AJP - Endocrinology and Metabolism, Vol 261, Issue 2 E177-E182, Copyright © 1991 by American Physiological Society

ARTICLES

Endothelin increases the synthesis and secretion of atrial natriuretic peptide in neonatal rat cardiocytes

D. G. Gardner, E. D. Newman, K. K. Nakamura and K. P. Nguyen
Department of Medicine, University of California, San Francisco 94143.

Endothelin (ET) effected a dose-dependent increment in atrial natriuretic peptide (ANP) secretion and ANP mRNA accumulation in neonatal rat atrial and ventricular cardiocytes but had no effect on the processing of the ANP prohormone to the mature ANP product. The secretagogue effect was not limited by cell density. Both basal and ET-dependent secretory activity were abrogated by the calmodulin antagonist calmidazolium but were unaffected by meclophenamate or pertussis toxin. The magnitude of the ET-dependent increment in ANP secretion was amplified by culturing the cells in a dynamically pulsating (vs. static) environment, implying an interaction between mechanical and agonist-mediated secretory stimuli in this system. ET also promoted immunoreactive ANP release from primary cultures of fetal rat hypothalamic cultures, suggesting that this regulatory function may be generally employed in ANP gene-expressing cells. These findings demonstrate that ET has parallel effects on ANP synthesis and secretion and support a role for this peptide in the regulation of local and circulating levels of the natriuretic hormone.

This article has been cited by other articles:

				N. Shimojo, S. Jesmin, S. Zaedi, T. Otsuki, S. Maeda, N. Yamaguchi, K. Aonuma, Y. Hattori, and T. Miyauchi Contributory role of VEGF overexpression in endothelin-1-induced cardiomyocyte hypertrophy Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H474 - H481. [Abstract] [Full Text] [PDF]

				Q. He and M. C. LaPointe Src and Rac Mediate Endothelin-1 and Lysophosphatidic Acid Stimulation of the Human Brain Natriuretic Peptide Promoter Hypertension, February 1, 2001; 37(2): 478 - 484. [Abstract] [Full Text] [PDF]

				K. Kuwahara, Y. Saito, M. Harada, M. Ishikawa, E. Ogawa, Y. Miyamoto, I. Hamanaka, S. Kamitani, N. Kajiyama, N. Takahashi, et al. Involvement of Cardiotrophin-1 in Cardiac Myocyte-Nonmyocyte Interactions During Hypertrophy of Rat Cardiac Myocytes In Vitro Circulation, September 7, 1999; 100(10): 1116 - 1124. [Abstract] [Full Text] [PDF]

				G. G. Nussdorfer, G. P. Rossi, L. K. Malendowicz, and G. Mazzocchi Autocrine-Paracrine Endothelin System in the Physiology and Pathology of Steroid-Secreting Tissues Pharmacol. Rev., September 1, 1999; 51(3): 403 - 438. [Abstract] [Full Text] [PDF]

				A. Matsuura, W. Yamochi, K.-i. Hirata, S. Kawashima, and M. Yokoyama Stimulatory Interaction Between Vascular Endothelial Growth Factor and Endothelin-1 on Each Gene Expression Hypertension, July 1, 1998; 32(1): 89 - 95. [Abstract] [Full Text] [PDF]