AJP - Endocrinology and Metabolism, Vol 238, Issue 2 186-E192, Copyright © 1980 by American Physiological Society
Secretory effects of gastrins on isolated perfused porcine pancreas
S. L. Jensen, J. F. Rehfeld, J. J. Holst, J. Fahrenkrug, O. V. Nielsen and O. B. Schaffalitzky de Muckadell
The effects of the four main forms of gastrin (component I, gastrin-34,
gastrin-17, and gastrin-14) on insulin, glucagon, and exocrine secretion
were measured on the isolated perfused porcine pancreas. All gastrins were
studied in concentrations ranging from 10(-11) to 10(-8) M. Depending on
the glucose concentration in the perfusate, all four gastrins increased
insulin or glucagon secretion in a dose-dependent manner in concentrations
above 10(-10) M. These concentrations are slightly above the arterial
concentrations in normal pig and man, but they correspond to gastrin
concentrations measured in patients with achlorhydria and gastrinomas. The
exocrine secretion was stimulated by all gastrins in a dose-dependent
manner. The lowest concentrations that stimulated flow rate significantly
were within the physiologic range, 10(-11) and 10(-10) M. All gastrins
induced maximal flow rate at a concentration of 10(-9) M. The sulfated form
of gastrin-17 had the greatest efficacy. The results indicate that all
gastrins may influence the exocrine secretion under normal conditions and
the endocrine secretion in diseases with endogenous hypergastrinemia.
