AJP - Endocrinology and Metabolism, Vol 238, Issue 2 116-E123, Copyright © 1980 by American Physiological Society
Sleep-promoting material from human urine and its relation to factor S from brain
J. M. Krueger, J. Bacsik and J. Garcia-Arraras
A sleep-promoting factor was extracted from human urine. Intraventricular
infusion of the purified material induced excess slow-wave sleep in rats
and rabbits for 5--10 h after the infusion. Chemical properties of the
urinary factor were similar to those of factor S derived from whole brains
of sleep-deprived goats, sheep, and rabbits. The behavior of the urinary
factor in two ion exchange chromatographic steps, high voltage
electrophoresis, gel-filtration, and ultrafiltration was similar to that of
factor S. Effects of the purified urinary factor on slow-wave sleep of rats
and rabbits were similar in time-course and duration to those of factor S
from brain. However, the factor obtained from human urine did not increase
the amplitude of cortical slow waves to the same extent as did factor S
from brains of sleep-deprived animals.
